The Zee Cine Award Best Actor in a Negative Role is chosen by a jury organized by Zee Entertainment Enterprises, and the winner is announced at the ceremony.
Kajol was the first woman to win this award.
The winners are listed below:-
| Year | Winner | Film |
|---|---|---|
| 2010 | Ronit Roy | Udaan |
| 2007 | Arjun Rampal | Om Shanti Om |
| 2006 | Saif Ali Khan | Omkara |
| 2005 | Nana Patekar | Apaharan |
| 2004 | John Abraham | Dhoom |
| 2003 | Rahul Dev | Footpath |
| 2002 | Ajay Devgan | Deewangee |
| 2001 | Aftab Shivdasani | Kasoor |
| 2000 | Sushant Singh | Jungle |
| 1999 | Ashutosh Rana | Sangharsh |
| 1998 | Ashutosh Rana | Dushman |
| 1997 | Kajol | Gupt: The Hidden Truth |